Our new Clear+ tissue clearing system is the one strategy that delipidates samples without any alter in morphology and with minimum impact on structural integrity.
Megatome is usually a vibrating microtome created to area a wide range of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With substantial blade vibrating frequency and minimized blade deflection, Megatome permits significant-throughput tissue sectioning with uniform surface profile, along with small tissue problems and knowledge decline.
Antibodies may possibly take weeks to diffuse as a result of only some millimeters of tissue, which has a steep labeling gradient from surface to Main.
Working the CLI directly from a Gradle activity is just not at present supported. A distribution must be produced via gradlew :j2d-cli:distZip to produce a zip file that contains anything necessary to run.
Clone the task or seize the most recent launch. Running the utility will differ a tad based upon how you retrieve the task.
Our preformulated EasyIndex Alternative raises and homogenize the refractive index of delipidated tissue samples, rendering them totally transparent. This permits gentle penetration to the sample and assures the acquisition of significant-resolution, in-focus image data.
Megatome is usually a novel microtome which allows for high-precision sectioning of a wide range of tissue samples – from organoids, to arrays of animal organs, to intact human brain hemispheres – with nominal tissue injury and data reduction.
eFLASH is actually a speedy tissue labeling method that permits for uniform entire-organ staining in 20 rounds of labeling.
Absolutely delipidate total megatomi.com mouse brains or comparably sized samples in just one day with SmartBatch+, or in one week with our passive clearing package.
--icon: Specify an icon for use for the docset. Should be a 32x32 PNG, but this Device isn't going to verify the file's content material. No icon will probably be employed if This is certainly omitted.
This will produce a docset named Sample in the current Listing. Docset generation is usually personalized with optional arguments:
SHIELD avoids the variability of hydrogel embedding and the information decline from PFA preservation, shielding specimens for several rounds of processing.
--displayName: Will established the name as revealed in Dash. This can be handy in case you create a docset with identify SampleProject but Show identify Sample Venture in its place. This placing will default to the value of --title if omitted.
Our novel Defend tissue preservation technique varieties intramolecular bonds making use of polyfunctional, flexible epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.